Device

Part:BBa_K766008:Design

Designed by: Yu Zhao   Group: iGEM12_Tsinghua   (2012-09-25)


cI protein controlled by lacI and IPTG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We want to control the expression of cI gene and construct some logic gate using this part.


Source

We clone cI gene from other parts and add lac operator and Plac promoter by PCR step by step.

References